Custom Model Generation
Yecuris FRG® KO mice offer a powerful advantage over other humanized mouse models. The platform can be transplanted with cells from any donor or species to yield an infinite number of phenotypic model variants. We’ve worked with hundreds of clients for over a decade to incorporate normal, super, and mutant donors for drug metabolism, NASH, metabolic disease, and infectious disease. Whether you’re looking to bridge data from previous experiments to a human-relevant model, or looking to create your own novel model, the FRG® KO platform is the ideal solution.
HepaSpecies Cross-Species Donor Comparisons Using FRG Mice
Xenotransplanted FRG®KO mice offer the ideal platform for accelerated preclinical testing
Streamline your process. The efficiency of gene therapy vectors can vary widely from one species to the next, making pre-clinical validation difficult and costly. Yecuris offers FRG®KO mice repopulated with hepatocytes from a variety of species, including human, mouse, NHP, and others. These models can accelerate the development of your liver-targeted gene therapy vector in a cost-effective manner.
Advantages of xenografted FRG KO mice include:
Larger cohorts compared with NHP studies
Dramatically reduced viral doses required
Simplified regulatory requirements
Compare data from multiple species side-by-side
Consistency from a single platform
No pre-existing vector immunity
Perform studies in your own vivarium, or at Yecuris
Common species for the FRG cross-species comparison include:
- Mouse or rat
- Dog or pig
- Non-human Primates: cynomolgus or rhesus
- Human donors: male vs. female
- Any combination of the above: mouse vs. cyno vs. human hepatocytes engrafted in the FRG mice
HepFinity™ Human Hepatocyte Expansion Services
Have you found that hepatocyte donors with genetic variants are becoming more and more difficult to procure? The FRG mice are often used as a bioreactor by taking a single vial of precious hepatocytes and allowing them to repopulate in vivo to generate 5X to 6X more hepatocytes. Here’s how it works.
- You send us your favorite lot (or vial) of cryopreserved hepatocytes
- We expand your rare or precious hepatocytes using the FRG KO mice
- The hepatocytes are isolated via perfusion, purified to remove any residual mouse hepatocytes, and prepared for delivery
- We ship the plateable fresh or cryopreserved human hepatocytes to your lab
Yecuris Custom Services
Custom Hepatocyte Transplantation
Donor Performance Monitoring
Custom donors are monitored in-house by quantifying human albumin over time as well as survival rates. These metrics are compared to Yecuris internal donors as a reference.